5 x superscript iv rt buffer  (Thermo Fisher)

Journal: iScience

Article Title: No substantial preexisting B cell immunity against SARS-CoV-2 in healthy adults

doi: 10.1016/j.isci.2022.103951

Figure Lengend Snippet:

Article Snippet: Subsequently, samples were incubated with a reverse-transcription master mix (RT mix) consisting of 3 μl of 5 x Superscript IV RT buffer (Thermofisher Scientific), 0.5 μl dNTPs (Thermo Fisher Scientific), 1 μl DTT (Thermo Fisher Scientific), 0.1 μl of RNasin (Promega), 0.1 μl of RNaseOut (Thermo Fisher Scientific), 2.05 μl of nuclease-free H 2 O and 0.25 μl of Superscript IV (Thermofisher Scientific) per well and incubated at 42°C for 10 min, 25°C for 10 min, 50 °C for 10 min and 94°C for 5 min.

Techniques: Virus, Clinical Proteomics, Recombinant, Reverse Transcription, Expressing, Suspension, Enzyme-linked Immunosorbent Assay, Clone Assay, Bioprocessing, Generated, Random Hexamer, Plasmid Preparation, Software